Life Science Lab Reagents, Cell cultrue Media, Serum
Enzymatic Modification of Native Chitin and Conversion to Specialty Chemical Products.
: Chitin is one of the most abundant biomolecules on Earth, occurs on the skin crustacean and fungal cell wall. While polysaccharides which threatens to pollute the coastal ecosystem in the form of a shell-collecting waste, it has the potential to be converted into a highly profitable derivatives with applications in medicine, biotechnology, and wastewater treatment, among others.
Traditionally this is carried out by the labor of aggressive chemicals, resulting in low quality while producing toxic by-products. In the last decade, the enzymatic conversion of chitin has improved, although still not at the same level of cost-effectiveness compared to traditional methods because of its multi-step character.
Another serious weakness of biotechnological approaches are highly ordered structures of chitin, which makes it almost impossible for most of the glycoside hydrolase to act on. So far, only 10 families Auxiliary activities (AA10), including polysaccharides monooxygenases lytic (LPMOs), known to hydrolyze chitin original stubborn, which spares expensive first step of chemical or mechanical pre-treatment to enlarge the surface of the substrate. The main advantage of the enzymatic conversion of chitin over conventional chemical methods are biocompability and, more prominently, higher product specificity, product quality, and the results of the process.
Products with Mw higher because there is no depolymerisation not specific besides the title properly defined and acetylation patterns can be generated. It gives a new toolset thousands of new chitin and chitosan derivatives, as physio-chemical properties can be modified to suit the desired application. This review aims to provide an overview of biotechnology tools currently at hand, as well as the challenges and the steps necessary to achieve long-term goals enzymatic conversion of native chitin into specialty chemical products.
Enzymatic Modification of Native Chitin and Conversion to Specialty Chemical Products.
Boost Exploit Potential Waste Food: Extraction, Purification and Characterization Renewable Specialty Chemicals of blackcurrants (Ribes nigrum L.).
natural dyes extracted from renewable botanical sources, particularly from industrial waste presses epicarp blackcurrant fruit. A process was developed that use acidified water extraction followed by solid phase extraction (SPE) purification step which enables the production of anthocyanin-rich extract the good results (ca. 2% w / w based on the dry weight of raw materials).
The components in the extract are extensively characterized by HPLC, mass spectrometry, IR, NMR, and UV-vis spectroscopy. HPLC confirmed the presence of four anthocyanins: delphinidin-3-O-rutinosida (45%), cyanidin-3-O-rutinosida (31%), and the corresponding glucoside in 16% and 8%, respectively.
Description: A monoclonal antibody from clone 1F5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is not conjugated.
Description: A monoclonal antibody from clone 1F5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with ATTO 390.
Description: A monoclonal antibody from clone 1F5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with ATTO 488.
Description: A monoclonal antibody from clone 1F5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with ATTO 565.
Description: A monoclonal antibody from clone 1F5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with ATTO 594.
Description: A monoclonal antibody from clone 1F5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with ATTO 633.
Description: A monoclonal antibody from clone 1F5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with ATTO 655.
Description: A monoclonal antibody from clone 1F5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with ATTO 680.
Description: A monoclonal antibody from clone 1F5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with ATTO 700.
Description: A monoclonal antibody from clone 1F5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with Alkaline Phosphatase.
Description: A monoclonal antibody from clone 1F5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with APC.
Description: A monoclonal antibody from clone 1F5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with APC/Cy7.
Description: A monoclonal antibody from clone 1F5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with Biotin.
Description: A monoclonal antibody from clone 1F5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with Dylight 350.
Description: A monoclonal antibody from clone 1F5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with Dylight 405.
Description: A monoclonal antibody from clone 1F5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with Dylight 488.
Description: A monoclonal antibody from clone 1F5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with Dylight 594.
Description: A monoclonal antibody from clone 1F5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with Dylight 633.
Description: A monoclonal antibody from clone 1F5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with FITC.
Description: A monoclonal antibody from clone 1F5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with HRP.
Description: A monoclonal antibody from clone 1F5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with PE/ATTO 594.
Description: A monoclonal antibody from clone 1F5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with PerCP.
Description: A monoclonal antibody from clone 1F5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with RPE.
Description: A monoclonal antibody from clone 1F5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with Streptavidin.
Description: A monoclonal antibody from clone 1F5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is not conjugated.
Description: A monoclonal antibody from clone 2B5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is not conjugated.
Description: A monoclonal antibody from clone 2B5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with ATTO 390.
Description: A monoclonal antibody from clone 2B5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with ATTO 488.
Description: A monoclonal antibody from clone 2B5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with ATTO 565.
Description: A monoclonal antibody from clone 2B5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with ATTO 594.
Description: A monoclonal antibody from clone 2B5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with ATTO 633.
Description: A monoclonal antibody from clone 2B5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with ATTO 655.
Description: A monoclonal antibody from clone 2B5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with ATTO 680.
Description: A monoclonal antibody from clone 2B5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with ATTO 700.
Description: A monoclonal antibody from clone 2B5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with Alkaline Phosphatase.
Description: A monoclonal antibody from clone 2B5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with APC.
Description: A monoclonal antibody from clone 2B5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with APC/Cy7.
Description: A monoclonal antibody from clone 2B5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with Biotin.
Description: A monoclonal antibody from clone 2B5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with Dylight 350.
Description: A monoclonal antibody from clone 2B5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with Dylight 405.
Description: A monoclonal antibody from clone 2B5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with Dylight 488.
Description: A monoclonal antibody from clone 2B5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with Dylight 594.
Description: A monoclonal antibody from clone 2B5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with Dylight 633.
Description: A monoclonal antibody from clone 2B5 against Human Metallothionein. The host species for the production of this antibody is Mouse. The antigen used for immunization is Pseudomonas aeruginosa Recombinant full length Pseudomonas aeruginosa Metallothionein protein. The antibody is tested and validated for WB, ELISA assays with the following recommended dilutions: WB (1:1000); ELISA (1:800). This MAb for Metallothionein is conjugated with FITC.
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In sequential liquid-liquid partition of the sample aqueous-organic post-SPE, anthocyanins monomer (54.7%) and anthocyanin polymer (18%) were found in a layer of water with 3- O-rutinosides of myricetin (3.1%) and quercetin (3.2%), while isopropylacetate achieve selective extraction of caffeic acid (3%), p-coumaric acid (5%), and myricetin (2.5%) and quercetin (3.2%) aglycons. 3- O-glucoside from myricetin (3.1%) and quercetin (2%), along with a p-coumarate nigrumin- (1%) and nigrumin ferulate (0.5%) are selectively extracted from the remaining water fractions using ethyl acetate. This allows the total quantification of the close of blackcurrant extract composition.
